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mouse ccl7 protein levels  (Cusabio)


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    Cusabio mouse ccl7 protein levels
    PCR Primers Used in This Study
    Mouse Ccl7 Protein Levels, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ccl7+protein+levels/pmc06943379-102-2-14?v=Cusabio
    Average 93 stars, based on 5 article reviews
    mouse ccl7 protein levels - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Intestinal Epithelial Chemokine (C-C Motif) Ligand 7 Overexpression Enhances Acetaminophen-Induced Hepatotoxicity in Mice"

    Article Title: Intestinal Epithelial Chemokine (C-C Motif) Ligand 7 Overexpression Enhances Acetaminophen-Induced Hepatotoxicity in Mice

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2019.09.009

    PCR Primers Used in This Study
    Figure Legend Snippet: PCR Primers Used in This Study

    Techniques Used:

    Acetaminophen (APAP) both induces hepatotoxicity and develops gut barrier defects and colon epithelial inflammation. C57BL/6J mice were treated with APAP for 3 and 24 hours, and the control group was treated with phosphate-buffered saline (PBS). A: Plasma alanine aminotransferase (ALT) levels at 24 hours after APAP. B: Fluorescein isothiocyanate–dextran 4 KDa (FD-4) level in the plasma at 3 hours and 24 hours after APAP. C: Fecal albumin content at 3 hours and 24 hours after APAP. D: Relative 16s/18s ratio in the liver. E: Occludin and claudin-4 protein levels in the colon at 3 hours and 24 hours after APAP. F: Representative immunofluorescence staining for occludin and claudin-4 in the colon at 3 hours and 24 hours after APAP. G: mRNA levels of cytokines and chemokines in the colon and colonocyte at 24 hours after APAP. The Black box indicates the CCL7 mRNA expression that was focused on for the following study. Data are expressed as means ± SEM. n = 6 to 8. *P < 0.05. Original magnification, ×400.
    Figure Legend Snippet: Acetaminophen (APAP) both induces hepatotoxicity and develops gut barrier defects and colon epithelial inflammation. C57BL/6J mice were treated with APAP for 3 and 24 hours, and the control group was treated with phosphate-buffered saline (PBS). A: Plasma alanine aminotransferase (ALT) levels at 24 hours after APAP. B: Fluorescein isothiocyanate–dextran 4 KDa (FD-4) level in the plasma at 3 hours and 24 hours after APAP. C: Fecal albumin content at 3 hours and 24 hours after APAP. D: Relative 16s/18s ratio in the liver. E: Occludin and claudin-4 protein levels in the colon at 3 hours and 24 hours after APAP. F: Representative immunofluorescence staining for occludin and claudin-4 in the colon at 3 hours and 24 hours after APAP. G: mRNA levels of cytokines and chemokines in the colon and colonocyte at 24 hours after APAP. The Black box indicates the CCL7 mRNA expression that was focused on for the following study. Data are expressed as means ± SEM. n = 6 to 8. *P < 0.05. Original magnification, ×400.

    Techniques Used: Control, Saline, Clinical Proteomics, Immunofluorescence, Staining, Expressing

    Chemokine (C-C motif) ligand (CCL7) overexpressed in intestinal epitheliums enhances acetaminophen (APAP)-induced hepatotoxicity. A and B: Vector construction for CCL7tgIEC mice generation. C:CCL7 mRNA levels in isolated intestinal epithelium cells. D: CCL7 protein levels in isolated intestinal epithelium cells. E: Plasma alanine aminotransferase (ALT) levels after 24 hours APAP treatment. F: Histopathological examination of hepatic hematoxylin and eosin staining (left panel, green dashed lines indicate necrotic areas) and quantification of the necrotic area after 24 hours of APAP treatment (right panel). Data are expressed as means ± SEM. n = 8 to 10. *P < 0.05. Original magnification, ×200. DSI, distal small intestine; PSI, proximal small intestine; WT, wild-type.
    Figure Legend Snippet: Chemokine (C-C motif) ligand (CCL7) overexpressed in intestinal epitheliums enhances acetaminophen (APAP)-induced hepatotoxicity. A and B: Vector construction for CCL7tgIEC mice generation. C:CCL7 mRNA levels in isolated intestinal epithelium cells. D: CCL7 protein levels in isolated intestinal epithelium cells. E: Plasma alanine aminotransferase (ALT) levels after 24 hours APAP treatment. F: Histopathological examination of hepatic hematoxylin and eosin staining (left panel, green dashed lines indicate necrotic areas) and quantification of the necrotic area after 24 hours of APAP treatment (right panel). Data are expressed as means ± SEM. n = 8 to 10. *P < 0.05. Original magnification, ×200. DSI, distal small intestine; PSI, proximal small intestine; WT, wild-type.

    Techniques Used: Plasmid Preparation, Isolation, Clinical Proteomics, Staining

    CCL7 overexpression in intestinal epithelial cells (IECs) leads to gut barrier dysfunction and promotes bacterial translocation into the liver. A: Fluorescein isothiocyanate–dextran 4 KDa (FD-4) level in the plasma. B: Fecal albumin (Alb) content. C: Occludin and claudin-4 protein levels in the colon. D: Representative immunofluorescence staining for occludin and claudin-4 in the colon. E: Electron microscopy of mice colon. Arrows indicate the connection between two epithelia cells. F: Western blotting for phosphorylated myosin light chain kinase (p-MLCK) in the colon. G: Plasma endotoxin level and relative 16s/18s ratio in the liver. H: mRNA levels of toll-like receptors (TLRs) in the liver. I: mRNA levels of key cytokines and chemokines in the liver. Data are expressed as means ± SEM. n = 4 to 6. *P < 0.05. Original magnification: ×400 (D); ×40,000 (E). WT, wild-type.
    Figure Legend Snippet: CCL7 overexpression in intestinal epithelial cells (IECs) leads to gut barrier dysfunction and promotes bacterial translocation into the liver. A: Fluorescein isothiocyanate–dextran 4 KDa (FD-4) level in the plasma. B: Fecal albumin (Alb) content. C: Occludin and claudin-4 protein levels in the colon. D: Representative immunofluorescence staining for occludin and claudin-4 in the colon. E: Electron microscopy of mice colon. Arrows indicate the connection between two epithelia cells. F: Western blotting for phosphorylated myosin light chain kinase (p-MLCK) in the colon. G: Plasma endotoxin level and relative 16s/18s ratio in the liver. H: mRNA levels of toll-like receptors (TLRs) in the liver. I: mRNA levels of key cytokines and chemokines in the liver. Data are expressed as means ± SEM. n = 4 to 6. *P < 0.05. Original magnification: ×400 (D); ×40,000 (E). WT, wild-type.

    Techniques Used: Over Expression, Translocation Assay, Clinical Proteomics, Immunofluorescence, Staining, Electron Microscopy, Western Blot



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    mouse ccl7 protein levels  (Cusabio)
    93
    Cusabio mouse ccl7 protein levels
    PCR Primers Used in This Study
    Mouse Ccl7 Protein Levels, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ccl7+protein+levels/pmc06943379-102-2-14?v=Cusabio
    Average 93 stars, based on 1 article reviews
    mouse ccl7 protein levels - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    PCR Primers Used in This Study

    Journal: The American Journal of Pathology

    Article Title: Intestinal Epithelial Chemokine (C-C Motif) Ligand 7 Overexpression Enhances Acetaminophen-Induced Hepatotoxicity in Mice

    doi: 10.1016/j.ajpath.2019.09.009

    Figure Lengend Snippet: PCR Primers Used in This Study

    Article Snippet: Human and mouse CCL7 protein levels were detected by an enzyme-linked immunosorbent assay kit (CUSABIO, Wuhan, China).

    Techniques:

    Acetaminophen (APAP) both induces hepatotoxicity and develops gut barrier defects and colon epithelial inflammation. C57BL/6J mice were treated with APAP for 3 and 24 hours, and the control group was treated with phosphate-buffered saline (PBS). A: Plasma alanine aminotransferase (ALT) levels at 24 hours after APAP. B: Fluorescein isothiocyanate–dextran 4 KDa (FD-4) level in the plasma at 3 hours and 24 hours after APAP. C: Fecal albumin content at 3 hours and 24 hours after APAP. D: Relative 16s/18s ratio in the liver. E: Occludin and claudin-4 protein levels in the colon at 3 hours and 24 hours after APAP. F: Representative immunofluorescence staining for occludin and claudin-4 in the colon at 3 hours and 24 hours after APAP. G: mRNA levels of cytokines and chemokines in the colon and colonocyte at 24 hours after APAP. The Black box indicates the CCL7 mRNA expression that was focused on for the following study. Data are expressed as means ± SEM. n = 6 to 8. *P < 0.05. Original magnification, ×400.

    Journal: The American Journal of Pathology

    Article Title: Intestinal Epithelial Chemokine (C-C Motif) Ligand 7 Overexpression Enhances Acetaminophen-Induced Hepatotoxicity in Mice

    doi: 10.1016/j.ajpath.2019.09.009

    Figure Lengend Snippet: Acetaminophen (APAP) both induces hepatotoxicity and develops gut barrier defects and colon epithelial inflammation. C57BL/6J mice were treated with APAP for 3 and 24 hours, and the control group was treated with phosphate-buffered saline (PBS). A: Plasma alanine aminotransferase (ALT) levels at 24 hours after APAP. B: Fluorescein isothiocyanate–dextran 4 KDa (FD-4) level in the plasma at 3 hours and 24 hours after APAP. C: Fecal albumin content at 3 hours and 24 hours after APAP. D: Relative 16s/18s ratio in the liver. E: Occludin and claudin-4 protein levels in the colon at 3 hours and 24 hours after APAP. F: Representative immunofluorescence staining for occludin and claudin-4 in the colon at 3 hours and 24 hours after APAP. G: mRNA levels of cytokines and chemokines in the colon and colonocyte at 24 hours after APAP. The Black box indicates the CCL7 mRNA expression that was focused on for the following study. Data are expressed as means ± SEM. n = 6 to 8. *P < 0.05. Original magnification, ×400.

    Article Snippet: Human and mouse CCL7 protein levels were detected by an enzyme-linked immunosorbent assay kit (CUSABIO, Wuhan, China).

    Techniques: Control, Saline, Clinical Proteomics, Immunofluorescence, Staining, Expressing

    Chemokine (C-C motif) ligand (CCL7) overexpressed in intestinal epitheliums enhances acetaminophen (APAP)-induced hepatotoxicity. A and B: Vector construction for CCL7tgIEC mice generation. C:CCL7 mRNA levels in isolated intestinal epithelium cells. D: CCL7 protein levels in isolated intestinal epithelium cells. E: Plasma alanine aminotransferase (ALT) levels after 24 hours APAP treatment. F: Histopathological examination of hepatic hematoxylin and eosin staining (left panel, green dashed lines indicate necrotic areas) and quantification of the necrotic area after 24 hours of APAP treatment (right panel). Data are expressed as means ± SEM. n = 8 to 10. *P < 0.05. Original magnification, ×200. DSI, distal small intestine; PSI, proximal small intestine; WT, wild-type.

    Journal: The American Journal of Pathology

    Article Title: Intestinal Epithelial Chemokine (C-C Motif) Ligand 7 Overexpression Enhances Acetaminophen-Induced Hepatotoxicity in Mice

    doi: 10.1016/j.ajpath.2019.09.009

    Figure Lengend Snippet: Chemokine (C-C motif) ligand (CCL7) overexpressed in intestinal epitheliums enhances acetaminophen (APAP)-induced hepatotoxicity. A and B: Vector construction for CCL7tgIEC mice generation. C:CCL7 mRNA levels in isolated intestinal epithelium cells. D: CCL7 protein levels in isolated intestinal epithelium cells. E: Plasma alanine aminotransferase (ALT) levels after 24 hours APAP treatment. F: Histopathological examination of hepatic hematoxylin and eosin staining (left panel, green dashed lines indicate necrotic areas) and quantification of the necrotic area after 24 hours of APAP treatment (right panel). Data are expressed as means ± SEM. n = 8 to 10. *P < 0.05. Original magnification, ×200. DSI, distal small intestine; PSI, proximal small intestine; WT, wild-type.

    Article Snippet: Human and mouse CCL7 protein levels were detected by an enzyme-linked immunosorbent assay kit (CUSABIO, Wuhan, China).

    Techniques: Plasmid Preparation, Isolation, Clinical Proteomics, Staining

    CCL7 overexpression in intestinal epithelial cells (IECs) leads to gut barrier dysfunction and promotes bacterial translocation into the liver. A: Fluorescein isothiocyanate–dextran 4 KDa (FD-4) level in the plasma. B: Fecal albumin (Alb) content. C: Occludin and claudin-4 protein levels in the colon. D: Representative immunofluorescence staining for occludin and claudin-4 in the colon. E: Electron microscopy of mice colon. Arrows indicate the connection between two epithelia cells. F: Western blotting for phosphorylated myosin light chain kinase (p-MLCK) in the colon. G: Plasma endotoxin level and relative 16s/18s ratio in the liver. H: mRNA levels of toll-like receptors (TLRs) in the liver. I: mRNA levels of key cytokines and chemokines in the liver. Data are expressed as means ± SEM. n = 4 to 6. *P < 0.05. Original magnification: ×400 (D); ×40,000 (E). WT, wild-type.

    Journal: The American Journal of Pathology

    Article Title: Intestinal Epithelial Chemokine (C-C Motif) Ligand 7 Overexpression Enhances Acetaminophen-Induced Hepatotoxicity in Mice

    doi: 10.1016/j.ajpath.2019.09.009

    Figure Lengend Snippet: CCL7 overexpression in intestinal epithelial cells (IECs) leads to gut barrier dysfunction and promotes bacterial translocation into the liver. A: Fluorescein isothiocyanate–dextran 4 KDa (FD-4) level in the plasma. B: Fecal albumin (Alb) content. C: Occludin and claudin-4 protein levels in the colon. D: Representative immunofluorescence staining for occludin and claudin-4 in the colon. E: Electron microscopy of mice colon. Arrows indicate the connection between two epithelia cells. F: Western blotting for phosphorylated myosin light chain kinase (p-MLCK) in the colon. G: Plasma endotoxin level and relative 16s/18s ratio in the liver. H: mRNA levels of toll-like receptors (TLRs) in the liver. I: mRNA levels of key cytokines and chemokines in the liver. Data are expressed as means ± SEM. n = 4 to 6. *P < 0.05. Original magnification: ×400 (D); ×40,000 (E). WT, wild-type.

    Article Snippet: Human and mouse CCL7 protein levels were detected by an enzyme-linked immunosorbent assay kit (CUSABIO, Wuhan, China).

    Techniques: Over Expression, Translocation Assay, Clinical Proteomics, Immunofluorescence, Staining, Electron Microscopy, Western Blot